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11.
Catch per unit effort (CPUE), length, weight and maturity data for Clarias gariepinus were collected during monthly gillnet surveys in the upper Okavango Delta between 2001 and 2009 to investigate their relationship with the annual flood-pulse. CPUE, condition factor (K) and the proportion of ripe-running fish (PRR) in the population followed a unimodal annual cycle that could be modelled using water temperature and flood-pulse hydrology. Increased CPUE during declining water levels was most likely a result of feeding migrations and aggregation behaviour. The observed increase in K during low floods in October and November preceded the increase in PRR, which increased mainly with increasing temperature but appeared less dependent on flow. This study provided quantitative evidence that the biology of fish in the Okavango Delta is mainly dependent on the annual flood regime and, therefore, that conservation efforts should be focused on maintaining natural flow patterns in the face of climate change and potential water extraction schemes upstream. 相似文献
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An A in equilibrium with G transition in exon III is known to differentiate alleles A and B of the cattle beta-lactoglobulin (BLG) gene. A BLG exon III fragment containing the transition site was amplified by the polymerase chain reaction. Temperature gradient gel electrophoresis (TGGE) was then used to detect this transition and hence to genotype cattle: the AT base-pair in allele A was readily distinguished from the GC base-pair of allele B. TGGE can be used to detect any single base-pair substitution, and thus is a powerful method of detecting genetic variability. 相似文献
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《Molecular & cellular proteomics : MCP》2023,22(1):100476
Cancer-derived extracellular vesicles (EVs) promote tumorigenesis, premetastatic niche formation, and metastasis via their protein cargo. However, the proteins packaged by patient tumors into EVs cannot be determined in vivo because of the presence of EVs derived from other tissues. We therefore developed a cross-species proteomic method to quantify the human tumor-derived proteome of plasma EVs produced by patient-derived xenografts of four cancer types. Proteomic profiling revealed individualized packaging of novel protein cargo, and machine learning accurately classified the type of the underlying tumor. 相似文献
14.
1. Worldwide, the excessive use of insecticides has resulted in field-evolved insecticide-resistant populations of diamondback moth (DBM), Plutella xylostella. A deltamethrin-resistant DBM population from the field was divided into two subpopulations in the laboratory. One population (S-strain) was maintained with no further exposure to insecticides, whereas the other population (R-strain) was maintained under a regime of intermittent selection with deltamethrin. 2. Individuals from both strains were reared at constant temperatures in the range 10–35 °C in the absence of deltamethrin, and the effects of rearing temperature on various traits were investigated. At the time of experimentation, the R-strain was 20-fold more resistant to deltamethrin than the S-strain. 3. Temperature differentially affected developmental time, adult life span, pupal weight, and fecundity of both strains. Although both strains laid eggs after being reared at 10 °C, few of these eggs were fertile. The R-strain developed significantly faster than the S-strain. The integrated performance of the S-strain and R-strain was greatest at 25 and 15 °C, respectively. 4. The present study provides important information on the complexities of the outcomes of the interactions between ectotherms and temperature. Specifically, temperature-trait relationships may not be unimodal, and ectotherm genotypes (in this case insecticide-resistance status) and abiotic stresses can interact with unpredictable outcomes. 5. Current models predicting DBM population dynamics and relative abundance in different locations do not consider different thermal biologies of different genotypes. The present study shows the dramatic effects of environment on many parameters used in these models and will help to enhance their accuracy, and thus their utility. 相似文献
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A rapid altitudinal range expansion in the pine processionary moth produced by the 2003 climatic anomaly 总被引:2,自引:0,他引:2
ANDREA BATTISTI MICHAEL STASTNY† EMILIANO BUFFO STIG LARSSON‡ 《Global Change Biology》2006,12(4):662-671
Climatic anomalies may produce, or accelerate, geographic range expansions of species limited by temperature or other climatic variables. Most such expansions are only temporary, before the prevailing climatic conditions drive the founder populations extinct. In contrast, here, we report a recent rapid shift of the range limit during the record hot summer of 2003 in southern Europe that has the potential to be both permanent, and to have important implications on species range dynamics in general. The winter pine processionary moth (Thaumetopoea pityocampa), an important pine defoliator whose larvae feed in colonies during the winter, is limited in its distribution by winter temperatures. In the last three decades, warmer winters have led to a gradual but substantial expansion of its range both latitudinally and altitudinally. In the summer of 2003, T. pityocampa underwent an extraordinary expansion to high elevation pine stands in the Italian Alps; its altitudinal range limit increased by one third of the total altitudinal expansion over the previous three decades. In an experiment, we found flight activity of newly emerged females to increase with temperature. By determining a threshold temperature for flight take‐offs under controlled conditions, we calculated that the nights above the threshold temperature were over five times more frequent, and considerably warmer, at the range limit in 2003 than in an average year. We therefore attribute the colonization of extreme, high‐elevation sites to increased nocturnal dispersal of females during the unusually warm night temperatures in June – August 2003. Importantly, the colonies established at extreme sites survived the winter and produced offspring in 2004, although the range did not expand further because of low night temperatures that year. We discuss several life‐history characteristics of T. pityocampa that maximize the likelihood of population persistence at the new range limit. As global warming continues and climatic anomalies are predicted to become more frequent, our results draw attention to the importance of extreme climatic events in the range formation of phytophagous insects. 相似文献
20.
Growth of 2659 Atlantic cod Gadus morhua aged 4 to 9 years examined in Placentia Bay, Newfoundland, peaked in most cases in June and was at a minimum in October or November. Water temperature, partial fullness index ( I P ) and gonado‐somatic index ( I G ) explained between 31 and 52% of the monthly variability in growth. Temperature and I P of capelin Mallotus villosus had significant effects on growth of all age groups and explained most of the variance for ages 6–8 and 4–5 years, respectively. The I P of large invertebrates (ages 4 to 7 years), sandlance ( Ammodytes sp. age 6 years) and demersal fishes (age 9 years) had age‐specific effects in the model. Overall, amphipods, decapods and echinoderms dominated the Atlantic cod diet in most seasons, but fish consumption by Atlantic cod was high in June and July, particularly on capelin. The rapid increase in somatic mass during June and July occurred despite cold water temperatures ( < 3° C at 50 m) and moderate to high gonado‐somatic index. The findings of this study suggest that when food was not a limiting factor, growth tended to increase even when Atlantic cod occupied colder waters, but when food was limiting, the opposite may have occured. 相似文献